Title
Evaluation of commercial cellulolytic enzymes for sugarcane bagasse hydrolysis
Authors
MARIO M. AGUIAR VIVIAN C. PIETROBON MARILIA MOURA DE SALLES PUPO NÁDIA HORTENSE TORRES JULIANA H. P. AMÉRICO GIANCARLO RICHARD SALAZAR-BANDA DANIEL P. SILVA REGINA TERESA ROSIM MONTEIRO and LUIZ F. ROMANHOLO FERREIRA1

Published Volume 52 Issue 7-8 July-August
Keywords biomass, cellulose, total sugars, cellulases, bagasse

Abstract
Conversion of plant biomass to glucose requires the synergistic effort of cellulolytic enzymes. Commercial cellulolytic enzymes (HPL, CL, P1 and P4) were used for enzymatic hydrolysis of sugarcane bagasse and the amount of total released sugars was quantified. Total cellulolytic activity and the required enzyme concentration were determined. Then, the enzymes were tested with regard to their efficiency in pretreating sugarcane bagasse in acidic and alkaline solutions, followed by a steam autoclave treatment. Upon total sugars quantification, it was possible to conclude that the most efficient bagasse treatment was an acidic pretreatment procedure with commercial enzyme P4.