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Title
Hydrolysis of Tilia japonica wood for production of a fermentable substrate
Authors
Takuya Yamaguchi and Masakazu Aoyama
Received
March 5, 2010
Published
Volume 44 Issue 7-8 July-August
Keywords
Tilia japonica, hydrolysis with dilute sulfuric acid, xylose
Abstract
To prepare a substrate for microbial conversion of xylose into xylitol, Tilia japonica wood was hydrolyzed with dilute sulfuric acid. When the reaction temperature was fixed at 121 °C, an optimum yield of xylose was obtained by treatment with 3% sulfuric acid, for 60 min. Both an increase in the sulfuric acid concentration and a prolonged residence time resulted in a decrease in the xylose yield. A fermentable substrate with a relatively high xylose concentration (57.0 g L-1) was obtained by hydrolysis with 3% sulfuric acid, at a liquid-to-solid ratio of 3 g g-1. During hydrolysis at elevated temperatures, certain undesired by-products were also generated, such as degradation products of solubilized sugars and lignin, which are potential inhibitors of microbial metabolism. These compounds were, however, successfully removed from the hydrolyzate by treatment with activated char.
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