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Title
Fungal pretreatment of oil palm empty fruit bunch: Effect of manganese and nitrogen
Authors
SRI HARMINI MIKAEL DJATI HUTOMO LUTFI BAHRUDDIN RIA MILLATI M. NUR CAHYANTO CLAES NIKLASON and MOHAMMAD J. TAHERZADEH
Received
September 11, 2012
Published
Volume 47 Issue 9-10 October-December
Keywords
oil palm empty fruit bunch, pretreatment, ligninolytic enzymes, glucosamine, fungal growth, Pleurotus floridanus
Abstract
Oil palm empty fruit bunch (OPEFB) was biologically pretreated using Pleurotus floridanus LIPIMC996 supplemented
with various concentrations of manganese and nitrogen and incubated for 35 days at 30°C, and enzyme activities of
manganese peroxidase (MnP) and laccase were examined. When OPEFB was supplemented with manganese, the
highest lignin reduction was achieved up to 25.0±5.6% at the addition of 200 µg Mn2+/g OPEFB.In addition, Pleurotus
floridanus LIPIMC996 grew best on OPEFB supplemented with 800 µg Mn2+/g OPEFB. When OPEFB was
supplemented with nitrogen, the highest lignin reduction was achieved up to 27.2± 3.5% at the addition of 20 mM
nitrogen. The best growth of Pleurotus floridanus was also achieved with the addition of 20 mM nitrogen. The addition
of nitrogen and manganese on OPEFB did not significantly affect the activity of MnP and laccase.
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