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Title
Potential applications of wastes from energy and forestry industry in plant tissue culture
Authors
CORNELIU TANASE IRINA VOLF SMARANDA VINTU ROBERT GRADINARU and VALENTIN I. POPA
Received
June 4, 2013
Published
Volume 47 Issue 7-8 July-August
Keywords
deuterium depleted water (DDW), spruce bark polyphenols (SB), callus maize, physiological parameters,
enzyme activities, polyphenol biosynthesis
Abstract
Two industrial by-products, deuterium depleted water (DDW) and polyphenolic extract from spruce wood bark (SB)
were tested as bioregulators for maize callus cultures. The effects of deuterium depleted water alone or in combination
with spruce bark extract on in vitro growth and development of callus maize plant were studied. Specific parameters,
including biomass accumulation, adventitious roots and aerial adventitious roots organogenesis, formation of new
callus, as well as activity of enzymes, such as peroxidase, superoxide dismutase, catalase, and total content of
polyphenolic compounds accumulated in callus have been closely monitored. The results have shown that the tested
solutions stimulated the fresh biomass accumulation, adventitious roots organogenesis and formation of new callus by
comparison with the control sample. The most significant stimulatory effect on callus biomass accumulation and total
content of polyphenolic compounds was observed when the deuterium depleted water, combined with spruce bark
polyphenolic extract, was used for the performed treatments.
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