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Title
Quantification of cellulose contents by transmission spectra of plant tissues
Authors
ZOOBIA BASHIR AQEEL AHMAD SHAZIA SHAFIQUE TEHMINA ANJUM SOBIYA SHAFIQUE and WAHEED AKRAM
Received
September 11, 2012
Published
Volume 47 Issue 7-8 July-August
Keywords
transmission spectrum, cellulose staining, colorimetric analysis, equation of line, area under line, percentage
error
Abstract
Determination of cellulose is a part of numerous scientific studies and provides an interlinked base for many
developmental and physiological variations among living entities. So, quick and accurate quantification of cellulose can
never be over-emphasized. The present study leads towards the development of a method for quantification of cellulose
contents under microscope. It is basically dependent upon transmitted color spectrum of plant tissues and assesses
accurate cellulose quantity with an error of ±0.46%. By adopting this method, inter- and intracellular variations in
cellulose concentrations can efficiently be determined by measuring variations in color intensity. Moreover, it
introduces a technique of physiological studies and also provides a base for development of similar formulas for other
biochemicals of living tissues.
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