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Title
Effect of different conditions on the average degree of polymerization of bacterial cellulose produced by Gluconacetobacter intermedius BC-41
Authors
QING-SHAN SHI JIN FENG WEN-RU LI GANG ZHOU AI-MEI CHEN YOU- SHENG OUYANG and YI-BEN CHEN
Received
July 15, 2012
Published
Volume 47 Issue 7-8 July-August
Keywords
Gluconacetobacter intermedius BC-41, average degree of polymerization, bacterial cellulose, viscosity
method, different conditions
Abstract
The average degree of polymerization (DP) of bacterial cellulose (BC), which was secreted by selected
Gluconacetobacter intermedius BC-41, was studied using the viscosity measurement method under five different sets
of conditions, with varying media, cultivation modes, fermentation time, carbon sources and surfactants. The DP of
BC produced in A9 medium was 22.9% higher than that produced in Schenk and Hildebrandt (SH) medium.
Meanwhile, there was not a significantly different DP under static and shaking culture conditions. However, the DP
was remarkably influenced by fermentation time. The BC produced with glycerin or xylose as carbon source
exhibited lower DP than that produced with glucose. Adding Tween 20 or Tween 80 in A9 medium had little effect on
the DP, while adding Triton X-100 decreased it. The results presented here would provide some experimental data for
BC production with different DP in practical applications.
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