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Title
Study on the modifications in the composition of sugars from lignocellulosic substrates exposed to the action of fungi
Authors
SIMONA IVANA ELISAVETA TULUCA and CRISTINA PIRVU DINU
Received
June 17, 2012
Published
Volume 47 Issue 5-6 May-June
Keywords
lignocellulosic substrates, lignolytic fungi, carbohydrate degradation
Abstract
The paper presents the results concerning the modifications in the sugar composition resulted from lignocellulosic
substrates subjected to the action of lignolytic fungi: Pleurotus ostreatus and Phanerochaete chrysosporium. The
substrates used were formed by various mixtures of corn cobs and oak tree leaves, oak tree sawdust and oak tree leaves,
and oak tree bark – oak tree leaves, all in ratios of 2:1 (marked as mixtures I, II and III). The evaluation of the sugar
composition of the substrates biodegraded by the above-mentioned fungi, for 30 days, was performed after hydrolysis,
using 1N sulfuric acid at 120 °C, for one hour, followed by sodium carbonate treatment until a pH of 5.8 was reached.
The quantitative determinations were performed through gas chromatography. The gathered data showed that the
highest degree of the heteropolysaccharide degradation corresponded to Pleurotus ostreatus, while the fungus
Phanerochaete chrysosporium cultivated under the same conditions degraded carbohydrates at a lower level. Also, a
large consumption of pentoses was recorded, evaluated through the variation of the xylose amounts, which rapidly
decreased in case of the Pleurotus ostreatus microorganism, in comparison with the substrate inoculated by
Phanerochaete chrysosporium.
Similarly, the hexoses consumption levels (mainly of glucose) were quite modest in the case of Pleurotus ostreatus and
quite significant for Phanerochaete chrysosporium.
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