|
Title
Elucidation of the bonds between cellulose and dehydrogenation polymer with Carbon-13 isotopic tracer method
Authors
HAITAO YANG XING ZHEN LAN YAO RUIJUN GU and YIMIN XIE
Received
May 22, 2012
Published
Volume 47 Issue 3-4 March-April
Keywords
dehydrogenation polymer, cellulose, carbon-13 isotopic tracer, coniferin, 13C NMR
Abstract
In this study, the DHP-cellulose complexes (DHPCC) were prepared from coniferin-[α13C] and cellulose. The
CP/MAS indicated that the linkage between lignin and cellulose was a benzyl ether bond. The DHPCC was
hydrolyzed with cellulase. The enzymatic hydrolyzates were fractionated into water-soluble and water-insoluble
fractions. The water-soluble fractions were fractionated by adsorption chromatography. Comparing the 13C NMR
spectra of the labeled water-insoluble fractions and LC-rich water-soluble fractions with those from the unlabeled
DHPCC, it was possible to observe significant enhancement in the signal intensities of the α-carbons. The main
substructures of the DHPCC were β-O-4, β-β, β-5, β-1 and coniferyl alcohol. Furthermore, β-O-4 units were linked
with cellulose at the α-position through benzyl ether bonds and acetal bonds.
Link
|
