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Title
Minimizing cellulase biosynthesis from cellulase-free xylanase production with Streptomyces Spp. P12-137 using optimization by response surface methodology
Authors
Gigi Coman and Gabriela Bahrim
Received
July 9, 2010
Published
Volume 45 Issue 3-4 March-April
Keywords
cellulose-free xylanase, Streptomyces, response surface methodology, media optimization,
Abstract
Response surface methodology and central composite design (CCD) were employed to optimize the biosynthesis medium for the production of cellulase-free xylanase by Streptomyces spp. P12-137 in submerged culture (SmF). The three independent variables were wheat bran, potassium nitrate and xylose concentrations. The statistical analyses of the results obtained over the studied range showed that wheat bran and potassium nitrate had a significant effect on minimizing cellulase production. The optimized biosynthesis medium containing 2.0 g% wheat bran, 0.2 g% KNO and 0.1 g% xylose, showed a decreasing 3 level of cellulose, with simultaneously increasing xylanase production. Cellulase-free xylanase can be used in several applications in the paper and pulp bleaching industry.
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