Title
Preparation of crystallinity tailored silk fibroin-sodium alginate based floating microbeads for nevirapine delivery
Authors
BHUPESH DIGAMBAR PATIL, SOPAN NAMDEV NANGARE and LAXMIKANT RAMVALLABH ZAWAR

Received April 13, 2023
Published Volume 57 Issue 5-6 May-June
Keywords silk fibroin, nevirapine, floating drug delivery, microbeads, crystallinity modulation, solvent annealing

Abstract
The present work anticipated crystallinity-tuned silk fibroin (SFIB)-sodium alginate floating microbeads (MB) as a candidate for nevirapine (NEV) sustained release. Briefly, crystallinity tuning was accomplished using solvent annealing. The changes in structural conformation of SFIB were validated using FTIR spectroscopy. Here, the tangent baseline method revealed changes in crystallinity of floating NEV-loaded SFIB-MB. Importantly, solvent annealing offers conversion of amorphous ‘α-helix’ to crystalline ‘β-sheet’ of SFIB, helping to modify drug release from the matrix of SFIB-sodium alginate. As well, NEV-loaded SFIB-MB demonstrated good floating profile. The NEV-loaded SFIB-MB with ethanol (ETH-6) annealing for 6 hours shows 25.853% drug release at 12 hours (pH = 1.2), compared to untreated NEV-loaded SFIB-MB (65.132%, 12 hours, log p < 0.0001). The release kinetics of batch ETH-6 revealed first-order release kinetics and Fickian diffusion (n = 0.468) was found to be the drug diffusion mechanism. Therefore, crystallinity-modified floating NEV-loaded SFIB-based MB will open a new door for modified drug delivery.